Exploring the Healthy Eye Microbiota Niche in a Multicenter Study

PUBLISHED

By Dr. Davide Borroni / MD PhD

Project aim
To explore and characterize healthy eye microbiota.

Execution
Samples were collected with an eSwab with 1 mL of Liquid Amies Medium (Copan Brescia, Italy). Following DNA extraction, libraries preparation, and amplification, PCR products were purified and end-repaired for barcode ligation. Libraries were pooled to a final concentration of 26 pM. Template preparation was performed with Ion Chef according to Ion 510, Ion 520, and Ion 530 Kit-Chef protocol. Sequencing of the amplicon libraries was carried out on a 520 or 530 chip using the Ion Torrent S5 system (Thermo Fisher; Waltham, MA, USA). Raw reads were analyzed with GAIA (v 2.02).

Results
We identified nine different ECSTs. A better understanding of healthy eye microbiota has the potential to improve disease diagnosis and personalized regimens to promote health.


Project Manager
Dr. Borroni Davide / MD PhD

Supervisor
Dr. Borroni Davide / MD PhD

Date
15/12/2022

Funding partners
Eyemetagenomics Ltd

Published on

Tagged in: Project

🇬🇧
🇮🇹
🇪🇸
Eyemetagenomics Ltd
71-75 Shelton Street, Covent Garden
London WC2H 9JQ, ENGLAND
LUCY does not claim to diagnose, treat, cure, or prevent any disease. The information on the LUCY website is provided for informational purposes only and with the understanding that LUCY is not engaged in rendering medical advice or recommendations. You should not rely on any information on the website to diagnose or treat a health problem or condition or replace consultations with qualified health care professionals to meet your individual needs. Always check with your doctor for answers to your personal medical questions.

Copyright © 2024 Eyemetagenomics Ltd. All rights reserved.
Cookie Policy