Exploring the Healthy Eye Microbiota Niche in a Multicenter Study

By Dr. Davide Borroni / MD PhD

Project aim
To explore and characterize healthy eye microbiota.

Execution
Samples were collected with an eSwab with 1 mL of Liquid Amies Medium (Copan Brescia, Italy). Following DNA extraction, libraries preparation, and amplification, PCR products were purified and end-repaired for barcode ligation. Libraries were pooled to a final concentration of 26 pM. Template preparation was performed with Ion Chef according to Ion 510, Ion 520, and Ion 530 Kit-Chef protocol. Sequencing of the amplicon libraries was carried out on a 520 or 530 chip using the Ion Torrent S5 system (Thermo Fisher; Waltham, MA, USA). Raw reads were analyzed with GAIA (v 2.02).

Results
We identified nine different ECSTs. A better understanding of healthy eye microbiota has the potential to improve disease diagnosis and personalized regimens to promote health.

Project Manager
Dr. Borroni Davide / MD PhD

Supervisor
Dr. Borroni Davide / MD PhD

Date
15/12/2022

Funding partners
Eyemetagenomics Ltd